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Practical validation of sorted fractions was carried out by xenotransplantation, the result of which was coupled with miRNA expression profiling to make stem mobile-associated miRNA expression profiles.
To determine no matter if miRNA are differentially expressed in LSC and HSC, we fractionated 16 AML individual samples and a few lineage-depleted (Lin–) wire blood (CB) samples making use of CD34 and CD38 into 4 populations and subjected Every single to world miRNA profiling; the stem cell content of every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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Decreasing the miR-126 amount impairs LSC maintenance in distinction to increasing typical hematopoietic stem cells.
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The LSC frequency was reduced in two of a few samples on 126KD (Figures 5C and S5D). Jointly, these findings propose that 126KD makes heterogeneous responses with LSC purpose and frequency minimized inside of a subset of AML sufferers.
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(E) Per cent BrdU incorporation into bulk cultures exhibiting proliferation of CTRL and 126OE transduced 8227 cells with time.
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